In order to investigate the effect of bovine serum albumin on in vitro growth and development of F1 hybrid mouse(C57BL×CBA) two cell stage embryos fertilized in vivo in simple(m-KRB) and complex(Ham's F-10) media, this study was performed and
the
results as follows are obtained.
1) When the early preimplantation in vitro growth and development to the stage of 4 cell or more was observed, in simple media the growth and development was suppressed significantly with an addition of bovine serum albumin and in complex media
the
growth and development was enhanced significantly with an addition of bovine serum albumin.
When the comparison was made between simple media with bovine serum albumin and complex media with bovine serum albumin, the growth and development in complex media with bovine serum albumin was significantly greater.
2) When the late preimplantation in vitro growth and development to the stage of blastocyst or hatched blastocyst was observed, in simple media the growth and development was suppressed significantly with an addition of bovine serum albumin and
in
complex media the growth and development was enhanced significantly with an addation of bovine serum seum albumin.
When the comparison was made between simple media with bovine serum albumin and complex media with bovine serum albumin, the growth and development in complex media with bovine serum albumin was significantly greater.
Judging from this, in preimplantation in vitro growth and development of two cell stage mouse(C57BL×CBA) embryos fertilized in vivo the media with additional nutrients and protein substances that might be much larger amount than usual would be
necessary.
However, as the preimplantation in vitro growth and development might be variant according to the strains of mouse and different stages of the growth and development every offort should be made to clearify the characteristics of preimplantation
in
vitro
growth and development among various animal species and different and different development stages.
So, therefore, human in vitro fertilization and embryo transfer program would be much progressed with an aid o such experimental researches.
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